Off-target effect of CWG-cPIP on gene expression of mouse Striatum

CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in the brain, CWG-cPIP (664 ug/kg) was intracerebroventricularly injected into mouse. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-injected mouse striatum 3 weeks later, mixing with ERCC RNA spike-in controls. Comparative gene expression profiling analysis of RNA sequencing data for the hippocampus of vehicle- or CWG-cPIP-treated C57BL/6J mice (n = 3 mice each).

CWG-cPIP靶向CAG/CTG（CWG）三核苷酸重复DNA，以重复序列长度依赖的方式抑制转录。为探究CWG-cPIP对大脑基因表达的脱靶效应，研究人员向小鼠脑室内注射剂量为664 μg/kg的CWG-cPIP。3周后，对经溶剂对照或CWG-cPIP注射的小鼠纹状体进行RNA测序分析，并加入ERCC RNA spike-in对照。本数据集针对经溶剂对照或CWG-cPIP处理的C57BL/6J小鼠海马体的RNA测序数据开展比较基因表达谱分析，每组各包含3只小鼠。