Genome-wide transcriptome profile of CYTOR (LINC00152)-silenced MDA-MB-231 breast cancer cell line

The transcriptional profile of MDA-MB-231 cells silenced for CYTOR (LINC00152, long intergenic non-protein coding RNA 152) was compared to LNA-control-treated MDA-MB-231 cells to identify potential CYTOR targets. MDA-MB-231 cells were treated with LNA targeting CYTOR or with LNA control (from Exiqon), and total RNA was obtained.

为鉴定潜在的CYTOR（LINC00152，基因间长链非编码RNA152）调控靶基因，本研究将CYTOR基因沉默的MDA-MB-231细胞的转录组谱，与经锁核酸（Locked Nucleic Acid，LNA）对照处理的MDA-MB-231细胞进行对比分析。实验中，将MDA-MB-231细胞分别用靶向CYTOR的LNA以及购自Exiqon公司的LNA对照进行处理，随后提取总RNA。