MAITs in HCC

Here we investigate the role of mucosal-associated invariant T (MAIT) cells in human and murine hepatocellular carcinoma (HCC). We isolated immune cells of syngeneic HCC cell line RIL-175 tumor-bearing or tumor-free livers and applied FACS sorting (CD45+ leukocytes) prior to 10X genomics based scRNA-seq. This is a complimentary dataset to the human scRNA-seq (deposited through dbGaP), highly multiplexed immunofluorescence microscopy using CODEX technology (deposited through TCIA) of paired patient samples. Overall design: Experimental setup: 7 weeks old female C57BL/6 mice were injected intrahepatically with syngeneic HCC cell line RIL-175 or left tumor-free. At d28 mice were sacrificed and liver and tumor-infiltrating MNCs were isolated after in vivo digestion. CD45+ sorted cells were subsequently subjected to single-cell RNA-sequencing.

本研究探讨黏膜相关恒定T细胞（mucosal-associated invariant T, MAIT）在人类及小鼠肝细胞癌（hepatocellular carcinoma, HCC）中的作用。我们分离了同基因肝细胞癌细胞系RIL-175荷瘤小鼠及无瘤小鼠的肝脏免疫细胞，先通过荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）分选出CD45+白细胞，随后开展基于10X Genomics的单细胞RNA测序（single-cell RNA-sequencing, scRNA-seq）。
本数据集是针对配对患者样本的人类单细胞RNA测序数据（已通过dbGaP提交）及采用CODEX技术的高多重免疫荧光显微镜成像数据（已通过TCIA提交）的补充数据集。
整体实验设计：实验方案为，选取7周龄雌性C57BL/6小鼠经肝内注射同基因肝细胞癌细胞系RIL-175构建荷瘤模型，或不予处理以保持无瘤状态；造模后第28天处死所有小鼠，经体内消化后分离肝脏及肿瘤浸润单个核细胞（mononuclear cells, MNCs），分选出的CD45+细胞随后被用于单细胞RNA测序。