Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

ABSTRACT Introduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10). Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans. Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR). Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection. Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.

摘要
引言：白色念珠菌（Candida albicans）是真菌性阴道炎最常见的致病菌。该类酵母菌可分泌由10个基因家族（SAP1-10）编码的分泌型天冬氨酸蛋白酶。
研究目的：本研究旨在分析外阴阴道来源白色念珠菌分离株中SAP1-7基因的携带情况。
材料与方法：本研究共纳入26株阴道分离的白色念珠菌，采用聚合酶链反应（PCR）检测其分泌型天冬氨酸蛋白酶基因SAP1-7。
结果：定植状态样本分离的白色念珠菌中，检出率最高的基因为SAP6（93.33%）；感染样本分离的菌株中SAP7检出率为100%。我们观察到外阴阴道定植与感染分离株的SAP1基因频率存在统计学差异（p=0.049）。
结论：外阴阴道来源白色念珠菌的SAP基因携带率较高。本研究结果提示SAP1参与了外阴阴道念珠菌病的感染过程。