Table_3_Genome-Wide Identification and Characterization of TCP Family Genes in Pak-Choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis var. communis].xlsx

The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) gene family, a kind of plant specific transcription factor, is essential for stress response, cell growth, and cell proliferation. However, the characterization of TCP family is still not clear in Pak-choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis var. communis]. In this study, genome-wide analysis of TCP gene family was performed and 26 TCP genes were identified in Pak-choi. Phylogenetic analysis demonstrated that the 26 BcTCPs were divided into two classes: Class I and Class II. Class II was further classified into two subclasses, CIN and CYC/TB1. The qPCR results suggested that most BcTCPs respond to abiotic stresses. The expressions of BcTCP3, BcTCP12, BcTCP21, and BcTCP22 were significantly changed under ABA and cold treatment. BcTCP3 and BcTCP12 were also up-regulated under osmotic treatment. Subcellular localization showed that BcTCP3 and BcTCP21 were located in the nucleus. Our results will facilitate revealing the functions and regulatory mechanisms of BcTCPs.

TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR（TCP）基因家族是一类植物特异性转录因子，在胁迫响应、细胞生长及细胞增殖过程中发挥关键作用。然而，目前关于小白菜（Brassica campestris（异名Brassica rapa）ssp. chinensis var. communis）中TCP家族的功能鉴定仍不明确。本研究通过全基因组分析，在小白菜中鉴定出26个TCP基因。系统发育分析显示，26个BcTCP基因可划分为两类：第I类与第II类，其中第II类可进一步分为CIN与CYC/TB1两个亚类。实时荧光定量PCR（qPCR）结果表明，多数BcTCP基因响应非生物胁迫；BcTCP3、BcTCP12、BcTCP21及BcTCP22的表达在脱落酸（ABA）与低温处理下发生显著变化，且BcTCP3与BcTCP12在渗透胁迫处理下同样呈现上调表达。亚细胞定位结果显示，BcTCP3与BcTCP21定位于细胞核。本研究结果将为解析BcTCP基因的功能及其调控机制提供重要参考。