Mycorrhizal effects on glomalin-related soil protein and chlorophyll contents in coffee plants in the Peruvian Amazon

ABSTRACT The inoculation of vegetatively propagated coffee plants with arbuscular mycorrhizal fungi (AMF) prior to field establishment may positively influence the plant growth and physiology. This study aimed to evaluate the AMF effects on the glomalin-related soil protein and chlorophyll contents in vegetatively propagated coffee plants inoculated in a greenhouse and transplanted to an open field, in the Peruvian Amazon. The experiment consisted of eight treatments, in a 2 × 4 factorial design, being two coffee varieties (Caturra and Pache) and four AMF inocula (control, Moyobamba, El Dorado and Huallaga). The inocula were collected from organic coffee crops and named according to the province from which they were collected. The mycorrhizal colonization and chlorophyll content were statistically higher in the plants inoculated with AMF, if compared to the non-inoculated plants, while the glomalin-related soil protein content ranged from 61.6 to 69.1 mg g-1 and showed no statistically significant differences among the inocula, although the Moyobamba inoculum showed to be numerically superior. The effect of the coffee variety was not statistically significant among the variables under study.

摘要：在大田定植前，对无性繁殖的咖啡植株接种丛枝菌根真菌（arbuscular mycorrhizal fungi，AMF），可能对植株生长及生理状态产生积极影响。本研究旨在评估经温室培育并移栽至秘鲁亚马逊地区大田的无性繁殖咖啡植株中，AMF对球囊霉素相关土壤蛋白与叶绿素含量的影响。本试验采用2×4因子设计，共设置8个处理：2个咖啡品种（卡杜拉（Caturra）与帕切（Pache）），以及4种AMF接种剂（对照组、莫约班巴（Moyobamba）、埃尔多拉多（El Dorado）与瓦拉加（Huallaga））。所有接种剂均采集自有机咖啡种植园，并以其采集所在省份命名。与未接种植株相比，接种AMF的植株其菌根定植率与叶绿素含量均显著提升；球囊霉素相关土壤蛋白含量介于61.6~69.1 mg·g⁻¹之间，尽管莫约班巴接种剂的对应数值略高，但各接种剂间未呈现统计学显著差异。咖啡品种对本研究所考察的各项变量均未产生统计学显著影响。