Results of ANOVA of soil physicochemical data for the factors treatment and time in control (NPK) and biochar (BC3N) treatments.

Data were analyzed by two-way mixed ANOVA. Data for protein, HMW-Norg and LMW-Norg were available only for one sampling date and therefore were analyzed by one-way ANOVA. Abbreviations: Corg, soil organic C; Ntot, total soil N; BD, soil bulk density; Grav. WC, gravimetric soil water content; WFPS, water filled pore space; AFPS, aire filled pore space; DOC, dissolved organic C; DON, dissolved organic N; NO3−, nitrate; NH4+, ammonium; FAA, total free amino acids; TDN, total dissolved N; HMW-Norg, high molecular weight organic N; LMW-Norg, low molecular weight organic N; DNA, soil DNA content; AOA/DNA, archaeal amoA copy numbers on DNA basis; AOA/soil, archaeal amoA copy numbers on dry soil basis; AOB/DNA, bacterial amoA copy numbers on DNA basis; AOB/soil, bacterial amoA copy numbers on dry soil basis.本研究采用双因素混合方差分析（two-way mixed ANOVA）对数据进行分析。针对蛋白质、高分子量有机氮（high molecular weight organic N, HMW-Norg）与低分子量有机氮（low molecular weight organic N, LMW-Norg）的相关数据仅在单次采样日期中可获取，故采用单因素方差分析（one-way ANOVA）进行统计分析。 缩写说明： Corg：土壤有机碳（soil organic C, Corg）； Ntot：土壤全氮（total soil N, Ntot）； BD：土壤容重（soil bulk density, BD）； Grav. WC：土壤质量含水量（gravimetric soil water content, Grav. WC）； WFPS：充水孔隙度（water filled pore space, WFPS）； AFPS：充气孔隙空间（aire filled pore space, AFPS）； DOC：溶解性有机碳（dissolved organic C, DOC）； DON：溶解性有机氮（dissolved organic N, DON）； NO3−：硝酸根（nitrate, NO3−）； NH4+：铵根（ammonium, NH4+）； FAA：总游离氨基酸（total free amino acids, FAA）； TDN：总溶解态氮（total dissolved N, TDN）； HMW-Norg：高分子量有机氮（high molecular weight organic N, HMW-Norg）； LMW-Norg：低分子量有机氮（low molecular weight organic N, LMW-Norg）； DNA：土壤DNA含量（soil DNA content, DNA）； AOA/DNA：以DNA为基准的古菌amoA基因拷贝数（archaeal amoA copy numbers on DNA basis, AOA/DNA）； AOA/soil：以干土为基准的古菌amoA基因拷贝数（archaeal amoA copy numbers on dry soil basis, AOA/soil）； AOB/DNA：以DNA为基准的细菌amoA基因拷贝数（bacterial amoA copy numbers on DNA basis, AOB/DNA）； AOB/soil：以干土为基准的细菌amoA基因拷贝数（bacterial amoA copy numbers on dry soil basis, AOB/soil）。