Transcriptome analysis about Litopenaeus vannamei in different dietary melatonin stress.

Melatonin (MT) is regarded as an antioxidant and immunostimulant that can efficiently scavenge free radicals and activate antioxidant enzymes. The aim of this study was to investigate the effects of dietary MT on the growth performance and immune function of the Pacific white shrimp (Litopenaeus vannamei). Six groups of L. vannamei were supplemented with dietary MT at 0, 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg levels for 2 months. RNA-Seq analysis was performed to obtain transcriptome data of the control group and the group supplemented with dietary MT at 82.7 mg/kg BW. In total, 1220 DEGs (799 up-regulated and 421 down-regulated) were identified.

褪黑素（Melatonin, MT）被视作一种抗氧化剂与免疫增强剂，可高效清除自由基并激活抗氧化酶系。本研究旨在探究日粮添加褪黑素对凡纳滨对虾（Litopenaeus vannamei，又称太平洋白虾）生长性能与免疫功能的影响。实验设置6组该对虾，分别饲喂添加了0、22.5、41.2、82.7、165.1及329.2 mg/kg剂量褪黑素的日粮，持续饲养2个月。随后对对照组及日粮添加82.7 mg/kg体重褪黑素的实验组开展RNA测序（RNA-Seq）分析，获取两组的转录组数据。最终共鉴定得到1220个差异表达基因（Differentially Expressed Genes, DEGs），其中799个基因上调表达，421个基因下调表达。