Properties of currents elicited by 10 µM choline plus 2 µM or 5 µM PNU-120596.

The results of experiments where 10 µM choline plus either 2 µM (top row) or 5 µM PNU-1205096 (bottom row) were applied to ACSF for at least 20 min and step-like current deviations in voltage-clamp or action potentials in current-clamp were recorded and analyzed. Significant differences were observed among the frequencies (p = 0.0001) and net charge (p = 0.0001) of step-like current deviations, but not the mean open time (p>0.67). Significant differences were also observed between the frequencies of action potentials (p&) Note that in the presence of 10 µM choline plus 2 µM PNU-120596, action potentials were observed in only 7 out of 13 CA1 pyramidal neurons.本数据集对应如下实验的结果：将10 μM胆碱（choline）与2 μM试剂（对应上排实验结果）或5 μM PNU-1205096（对应下排实验结果）共同加入人工脑脊液（ACSF，Artificial Cerebrospinal Fluid）中孵育至少20分钟，随后采用膜片钳技术，分别在电压钳（voltage-clamp）模式下记录阶梯样电流偏差（step-like current deviations）、在电流钳（current-clamp）模式下记录动作电位（action potentials），并对采集到的信号进行分析。对阶梯样电流偏差的统计分析显示，其频率（p=0.0001）与净电荷量（p=0.0001）存在显著组间差异，但平均开放时长无显著组间差异（p>0.67）。动作电位的频率同样存在显著组间差异（原文标注为p&）。值得注意的是，当使用10 μM胆碱与2 μM PNU-120596共同处理时，13个CA1锥体神经元（CA1 pyramidal neurons）中仅7个可记录到动作电位。