Sampling design for mRNA sequencing.

Parental fishes crossed to produce larvae for sequencing were either wild-caught (F0) or lab-raised over n generations (indicated by Fn). Individuals were sampled eight days post fertilization (dpf), 8–10 dpf, or 17–20 dpf.用于杂交制备测序所需幼鱼的亲本鱼类，可分为两类：一类为野外捕获个体（F0），另一类为经n代实验室繁育的个体（以Fn标注）。实验个体的采样时间分别为受精后8天（days post fertilization，简称dpf）、受精后8~10天，以及受精后17~20天。