Role of AcsR in expression of the acetly-CoA synthetase gene in Vibrio vulnificus. Vibrio vulnificus

VarS/A is one of the global factors regulating diverse aspects of metabolism and virulence of bacteria including pathogenic Vibrio spp. An experiment to identify VarS/A-regulon in V. vulnificus revealed that a putative LuxR-type transcriptional regulator was down-regulated in ΔvarA mutant. To investigate the roles of this regulatory cascade from VarS/A to a LuxR-type regulator in V. vulnificus, the target gene regulated by a LuxR-regulator was identified and its expression was characterized. Overall design: Four-condition experiment, Mo6 vs acsR, Mo6 vs VarA. Biological replicates: 9 wild-type strain, 4 acsR deletion mutant strain, 5 varA deletion mutant strain, independently grown and harvested. Please note that 'wild-type Mo6 vs acsR mutant replicate 1' sample was hybridized to the 30K platform chip while the others were hybridized to the 15K platform Therefore, the 'MO6 vs acsR_1.gpr' has 30,755 data rows (while the other files have 16,355). The 30K platform chip is made of duplication of the 15K platform chip with the same probes.

VarS/A是调控包括致病性弧菌属（pathogenic Vibrio spp.）在内的细菌代谢与毒力多方面进程的全局调控因子之一。针对创伤弧菌（V. vulnificus）中VarS/A调控子（regulon）开展的鉴定实验发现，推定的LuxR型转录调控因子（LuxR-type transcriptional regulator）在ΔvarA突变株（ΔvarA mutant）中表达下调。为探究该从VarS/A到LuxR型转录调控因子的调控级联在创伤弧菌中的功能，本研究鉴定了该LuxR型调控因子所靶向的靶基因，并对其表达特征进行了表征。
实验整体设计：本实验为四组对照设计，分别为Mo6与acsR突变株、Mo6与VarA突变株。
生物学重复设置：野生型菌株9份、acsR缺失突变株4份、varA缺失突变株5份，所有样本均经独立培养并收获。
请注意，"野生型Mo6与acsR突变株重复1"样本采用30K平台芯片进行杂交，其余样本均采用15K平台芯片进行杂交。因此，"MO6 vs acsR_1.gpr"文件包含30755条数据行（其余文件均为16355条）。30K平台芯片由15K平台芯片的探针重复复制得到，二者探针序列完全一致。