Data Sheet 2_Potential prognostic biomarkers in restenosis of peripheral arteries identified through comprehensive ceRNA network analysis.csv

Peripheral artery disease (PAD) is one of the leading causes of amputation and mortality worldwide, with restenosis being a common complication following vascular interventional therapy that significantly impacts patient prognosis. This study aims to identify potential biomarkers with clinical and prognostic significance for restenosis of peripheral arteries (RPA). To achieve this, RNA expression levels in stenotic vascular tissues from healthy controls and patients with RPA were integrated and analyzed, identifying a growing number of differentially expressed RNAs for the construction of a competitive endogenous RNA (ceRNA) network for prognostic analysis. The analysis revealed that the RNA expression patterns of samples from patients with RPA were significantly different compared with those of healthy controls. Consequently, the resulting differentially expressed RNAs were used to construct a ceRNA network. Through bioinformatics analysis, a complex regulatory network composed of multiple ceRNA axes was established. The findings indicate that key mRNAs in the network play critical roles in various biological functions. Given the prognostic significance of these six differentially expressed genes in RPA, real-time quantitative fluorescence PCR (qRT-PCR) analysis was performed. These results suggest that these candidate key differentially expressed genes are potential prognostic biomarkers for RPA, and their corresponding mRNAs may serve as potential therapeutic targets.

外周动脉疾病（Peripheral artery disease, PAD）是全球范围内引发截肢与死亡的主要病因之一，而再狭窄是血管介入治疗后常见的并发症，会显著影响患者预后。本研究旨在筛选兼具临床与预后价值的外周动脉再狭窄（Restenosis of peripheral arteries, RPA）潜在生物标志物。为达成研究目标，本研究整合并分析了健康对照及外周动脉再狭窄患者的狭窄血管组织中的RNA表达水平，筛选出大量差异表达RNA，用于构建内源竞争RNA（competitive endogenous RNA, ceRNA）网络以开展预后分析。分析结果显示，外周动脉再狭窄患者的样本RNA表达模式与健康对照存在显著差异。据此，研究人员利用所得差异表达RNA构建了ceRNA网络。通过生物信息学分析，本研究构建了由多条ceRNA轴构成的复杂调控网络。研究发现，网络中的关键信使RNA（messenger RNA, mRNA）在多种生物学功能中发挥关键作用。鉴于这6个差异表达基因在外周动脉再狭窄中具有预后价值，本研究开展了实时定量荧光聚合酶链式反应（real-time quantitative fluorescence PCR, qRT-PCR）验证。上述结果表明，这些候选关键差异表达基因可作为外周动脉再狭窄的潜在预后生物标志物，其对应的信使RNA也有望成为潜在治疗靶点。