Rhipicephalus sanguineus amplicon sequencing

Multiplexed amplicon short-read sequences of four phylogenetically informative mitochondrial (COI, 12S, and 16S) and nuclear (ITS2) loci, and five loci targeting regions encompassing common genetic mutations in the voltage-gated sodium channel (VGSC) and GABA-gated chloride channel (GABA-Cl) genes associated with synthetic pyrethroid (SP) and dieldrin/fipronil acaricide resistance. Two separate primer pairs capture the region of the domain III segment 6 of the VGSC gene containing the single point mutation (T2134C) previously associated with resistance to SPs in Rh. sanguineus s.l. from the US. Also included is a 199bp region of domain II segment 6 of the VGSC gene encompassing the single SNP associated with the SP resistance substitution L1014F, and a 167bp region of domain II segment 4-5 that encompasses regions containing eight other known mutations associated with SP resistance in a variety of arthropod species, including Rh. sanguineus s.l. Also included is a 231bp region of the GABA-Cl gene that contains several mutations associated with dieldrin/fipronil resistance in Drosophila spp. and Rh. microplus.

本数据集包含多重扩增子短读长测序序列，涵盖4个具备系统发育信息价值的基因座：线粒体基因座（COI、12S rRNA、16S rRNA）与核基因座（ITS2）；以及5个靶向特定区域的基因座，这些区域覆盖电压门控钠通道（voltage-gated sodium channel, VGSC）和γ-氨基丁酸门控氯离子通道（GABA-gated chloride channel, GABA-Cl）基因内的常见遗传突变，上述两类基因的突变与合成拟除虫菊酯（synthetic pyrethroid, SP）及狄氏剂、氟虫腈两类杀螨剂的抗药性相关。两组独立引物对可扩增VGSC基因第Ⅲ结构域第6区段的区域，该区域包含单点突变T2134C，该突变此前已被证实与美国境内血红扇头蜱复合种（Rh. sanguineus s.l.）对SP的抗药性相关。此外还包含VGSC基因第Ⅱ结构域第6区段的199bp区域，该区域涵盖与SP抗药性替换突变L1014F相关的单核苷酸多态性（single nucleotide polymorphism, SNP）；以及第Ⅱ结构域4-5区段的167bp区域，该区域覆盖在包括Rh. sanguineus s.l.在内的多种节肢动物中与SP抗药性相关的其余8种已知突变位点。另外还包含GABA-Cl基因的231bp区域，该区域存在果蝇属（Drosophila spp.）与微小牛蜱（Rh. microplus）中与狄氏剂、氟虫腈抗药性相关的多种突变位点。