S100A8 treatment on gene expression of human umbilical vascular endothelial cells.. Homo sapiens

Previous studies showed that S100A8 and S100A9 are involved in neovascularization as well as in tumor development. At high concentrations, S100A8 and S100A9 cause inflammatory response or apoptosis mediated damage in vascular endothelial cells. But the effect of low concentrations of such proteins on endothelial cells remains unknown. This assay was performed to screen for genes that are involved in the response of Human Unbilican Vascular Endothelial Cells to low concentrations of S100A8. Overall design: Human Umblical Vascular Endothelial Cells (HUVEC) were cultuered and treated with 10ug/mL S100A8 proteins for 4 or 24 hours. Gene profiling was carried out using two-color microarray. Two-condition experiment, S100A8 treatment vs. non-treatment. Two time points: 4 hours and 24 hours. Biological replicates at each time point: 3 control replicates, 3 treatment replicates.

既往研究表明，S100A8与S100A9参与血管新生以及肿瘤发生发展过程。当浓度较高时，S100A8与S100A9会引发血管内皮细胞产生炎症反应或凋亡介导的损伤；但低浓度此类蛋白对内皮细胞的作用仍未明确。本实验旨在筛选参与低浓度S100A8刺激下人脐静脉内皮细胞（Human Umbilical Vascular Endothelial Cells，HUVEC）应答反应的基因。
总体实验设计：将人脐静脉内皮细胞（HUVEC）培养后，用10μg/mL的S100A8蛋白分别处理4小时与24小时；采用双色微阵列（two-color microarray）进行基因表达谱分析。本实验为双条件对照实验，即S100A8处理组与未处理组，设置两个时间节点：4小时与24小时；每个时间点设置生物学重复：对照组与处理组各3次重复。