Genome-wide Promoter Mapping of Histone H3 Lysine 4 and Lysine 27 Methylation in Preimplantation Embryos

First lineage specification in the mammalian blastocyst embryo leads to formation of the inner cell mass (ICM) and trophectoderm (TE), which respectively give rise to the embryo proper and extraembryonic tissues. We show histone methylation asymmetry on promoters in the first two developmental lineages, and highlight epigenetic skewing associated with derivation of embryonic stem (ES) cells. Comparison of histone methylation patterns on promoters in the ICM, TE and in ES cells derived from ICM. ChIP-chip experiments using anti-H3K4me3 or anti-H3K27me3 antibodies. Two or three replicates per sample.

哺乳动物囊胚的首次谱系特化会形成内细胞团（inner cell mass, ICM）与滋养外胚层（trophectoderm, TE），二者分别分化为胚胎本体与胚外组织。本研究揭示了前两个发育谱系中启动子区域的组蛋白甲基化不对称性，并重点阐述了与胚胎干细胞（embryonic stem, ES细胞）建系相关的表观遗传偏倚。本数据集包含对ICM、TE以及由ICM衍生得到的ES细胞的启动子组蛋白甲基化模式的比较分析；实验采用抗H3K4me3或抗H3K27me3抗体开展染色质免疫沉淀芯片（ChIP-chip）检测，每个样本设置2至3次生物学重复。