Single cell gene expression, immune receptor and cell surface marker expression profiling of Spike-specfic splenic T and B cells of immunized mice

Spike-specific T and B cells from splenocytes of immunized mice were profiled with 10x sequencing for gene expression (Chromium Next Automated GEM 5’ v2 kit), cell surface marker (Chromium Automated 5’ Feature Barcode kit) and immune receptor (Chromium Automated Mouse BCR/TCR Amplification and Library Construction kit) Splenocytes from immunized mice were FACS sorted with fluorescently-labelled Spike protein tetramer or Spike pMHC tetramer for antigen specific T and B cells for 10x single cell profiling Please note that the processed data files generated from both *GEX and *CITE samples are linked to the corresponding *GEX sample records.

本研究针对免疫小鼠脾脏淋巴细胞中的刺突蛋白（Spike）特异性T、B淋巴细胞，采用10x单细胞测序技术开展多维度表征分析，涵盖基因表达（使用Chromium Next Automated GEM 5’ v2试剂盒）、细胞表面标志物（使用Chromium Automated 5’ Feature Barcode试剂盒）以及免疫受体（使用Chromium Automated Mouse BCR/TCR Amplification and Library Construction试剂盒）三个检测方向。本研究通过荧光标记的刺突蛋白四聚体或刺突蛋白-pMHC四聚体，对免疫小鼠脾脏淋巴细胞开展荧光激活细胞分选（FACS）以富集抗原特异性T、B淋巴细胞，用于后续10x单细胞测序分析。请注意：由*GEX与*CITE样本生成的处理后数据文件，均关联至对应的*GEX样本记录。