Real-Time PCR of validated genes for identification of biomarkers in selected tissue samples from fish treated with DES (5ug/L) for 72 hours.

*Not in the Discriminatory Gene Sets.**Known Estrogen-responsive genes.***All values shown are significant (P-value?Inconsistent with whole fish real-time PCR validated data.NS: Not Significant (P-value>0.05).#Confirmed as robust biomarker genes based on their significant deregulation in >50% tissues examined.* 未纳入差异基因集（Discriminatory Gene Sets）。** 已知雌激素应答基因。** 所有展示的数值均具有统计学显著性（P值？与全鱼实时荧光定量PCR（real-time PCR）验证数据不一致。NS：无统计学显著性（P值>0.05）。# 基于在所检测的超过50%的组织中呈现显著表达失调，该类基因被证实为稳健的生物标志物基因（biomarker genes）。