Genome-wide maps of FOXK2 binding sites in ovarian cancer cell line OVCAR5 and expression profile of FOXK2 knockdown OVCAR5 cells (ChIP-seq). Genome-wide maps of FOXK2 binding sites in ovarian cancer cell line OVCAR5 and expression profile of FOXK2 knockdown OVCAR5 cells (ChIP-seq)

This study characterized the genome-wide binding of FOXK2 in ovarian cancer (OC) cell OVCAR-5 and investigated the expression profile of OVCAR5 cells with FOXK2 knocking-down. Overall design: Two datasets are included: 1) Examined transcriptome of OVCAR5 cells transduced with control shRNA (shCtrl) and FOXK2-targeting shRNA (shFOXK2) in OVCAR5 cells (in biological duplicates); 2) Determined the genome-wide bindng and occupancy profile of FOXK2 in OVCAR5 cells by ChIP-seq (in biological duplicates).

本研究对卵巢癌（ovarian cancer, OC）细胞OVCAR-5中FOXK2的全基因组结合特征进行了系统表征，并探究了FOXK2敲低的OVCAR5细胞的基因表达谱。整体实验设计：本数据集包含两个数据集：1）针对转染对照短发卡RNA（short hairpin RNA, shRNA，shCtrl）及靶向FOXK2的短发卡RNA（shFOXK2）的OVCAR5细胞开展的转录组测序分析（设置两组生物学重复）；2）通过染色质免疫沉淀测序（chromatin immunoprecipitation sequencing, ChIP-seq）测定的OVCAR5细胞中FOXK2的全基因组结合与染色质占据谱（设置两组生物学重复）。