RNA-Seq based gene expression profiling of mouse epicardial-cells with Meis1-Meis2 double KO or wild-type genotypes.

The goal of this study was to compare the gene expression profile, using RNA-seq technology, of mouse epicardial-cells with Meis1-Meis2 double KO or wild-type genotypes. Epicardial cells, isolated from WT or Meis1-Meis2 double KO mice, were used to extract poly A RNA with the aim of studying the effect of the mutations on gene expression profiles, using RNA-Seq technology.

本研究旨在通过RNA测序（RNA-seq）技术，对比携带Meis1-Meis2双基因敲除（double knockout, KO）与野生型基因型的小鼠心外膜细胞的基因表达谱。研究人员从野生型（wild-type, WT）或Meis1-Meis2双基因敲除小鼠中分离心外膜细胞，提取其聚腺苷酸RNA，旨在借助RNA-seq技术探究该双基因突变对基因表达谱的影响。