Host transcriptome response to SARS-CoV2 infection

HEK293T stable express hACE2 was infected by SARS-CoV-2 (WA1) and RNA-seq was performed to profile host gene expression in response to SARS-COV-2 infection Overall design: Both mock and infection samples were duplicated, infected at MOI=1 for three days

本数据集以稳定表达hACE2（human angiotensin-converting enzyme 2，血管紧张素转换酶2）的HEK293T细胞为实验对象，以严重急性呼吸综合征冠状病毒2（SARS-CoV-2）WA1株感染细胞后，通过RNA测序（RNA-seq）解析宿主基因在病毒感染后的表达谱，以探究宿主对新冠病毒感染的应答反应。实验整体设计：空白对照（mock）组与感染组均设置生物学重复样本，以感染复数（MOI，multiplicity of infection）=1的比例感染细胞，并于感染后培养3天。