Br soaked lysozyme datasets collected with the mixed scheme implemented in HITO in the ZOO system

Bromide soaked lysozyme crystals were used as standard crystals for evaluation of the automated data collection, ZOO, developed at SPring-8 BL32XU. Crystals those sizes ranged 10-100 µm were cryocooled together in the same cryoloop and processed 'mixed scheme' implemented in the ZOO system. Beam size : 10(H) x 15(V) µm² After 2D raster scanning the loop, HITO detects 1 crystals for full rotation helical, 7 crystals for partial helical, and 62 crystals for multiple small wedge schemes, respectively. A full rotation helical, partial helical and small wedge datasets contain total oscillation of 60 deg, 40 deg and 5 deg. respectively. The crystals belong to space group P4₃2₁2 with mean unit cell parameters a=b=79.1, c=37.5. Data was processed up to 1.50 Å resolution in the published result (Hirata et al. in preparation) using KAMO. <em><strong>&lt;Available data&gt;</strong></em> # Beamline: SPring-8 BL32XU # wavelength 0.9000 A # Detector : EIGER X 9M # ZOO mode: mixed scheme (HITO) ##################################################### # Multiple small wedge datasets # 'CPS1991-05-multi_master.h5' manages 62 datasets ##################################################### # 5.0 deg. / datasets # 0.1 deg. / frame # 50 frames / datasets # 100 frames in each data file ##################################################### hito-CPS1991-05//data/CPS1991-05-multi_data_000001.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000002.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000003.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000004.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000005.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000006.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000007.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000008.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000009.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000010.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000011.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000012.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000013.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000014.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000015.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000016.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000017.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000018.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000019.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000020.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000021.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000022.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000023.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000024.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000025.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000026.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000027.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000028.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000029.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000030.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000031.h5 hito-CPS1991-05//data/CPS1991-05-multi_master.h5 ##################################################### # 'cry-00-??_master.h5' are for 'partial helical' datasets ##################################################### # 40.0 deg. / datasets # 0.1 deg. / frame # 400 frames / datasets # 100 frames in each data file ##################################################### hito-CPS1991-05//data/cry-00-00_data_000001.h5 hito-CPS1991-05//data/cry-00-00_data_000002.h5 hito-CPS1991-05//data/cry-00-00_data_000003.h5 hito-CPS1991-05//data/cry-00-00_data_000004.h5 hito-CPS1991-05//data/cry-00-00_master.h5 hito-CPS1991-05//data/cry-00-01_data_000001.h5 hito-CPS1991-05//data/cry-00-01_data_000002.h5 hito-CPS1991-05//data/cry-00-01_data_000003.h5 hito-CPS1991-05//data/cry-00-01_data_000004.h5 hito-CPS1991-05//data/cry-00-01_master.h5 hito-CPS1991-05//data/cry-00-02_data_000001.h5 hito-CPS1991-05//data/cry-00-02_data_000002.h5 hito-CPS1991-05//data/cry-00-02_data_000003.h5 hito-CPS1991-05//data/cry-00-02_data_000004.h5 hito-CPS1991-05//data/cry-00-02_master.h5 hito-CPS1991-05//data/cry-00-03_data_000001.h5 hito-CPS1991-05//data/cry-00-03_data_000002.h5 hito-CPS1991-05//data/cry-00-03_data_000003.h5 hito-CPS1991-05//data/cry-00-03_data_000004.h5 hito-CPS1991-05//data/cry-00-03_master.h5 hito-CPS1991-05//data/cry-00-04_data_000001.h5 hito-CPS1991-05//data/cry-00-04_data_000002.h5 hito-CPS1991-05//data/cry-00-04_data_000003.h5 hito-CPS1991-05//data/cry-00-04_data_000004.h5 hito-CPS1991-05//data/cry-00-04_master.h5 hito-CPS1991-05//data/cry-00-05_data_000001.h5 hito-CPS1991-05//data/cry-00-05_data_000002.h5 hito-CPS1991-05//data/cry-00-05_data_000003.h5 hito-CPS1991-05//data/cry-00-05_data_000004.h5 hito-CPS1991-05//data/cry-00-05_master.h5 hito-CPS1991-05//data/cry-00-06_data_000001.h5 hito-CPS1991-05//data/cry-00-06_data_000002.h5 hito-CPS1991-05//data/cry-00-06_data_000003.h5 hito-CPS1991-05//data/cry-00-06_data_000004.h5 hito-CPS1991-05//data/cry-00-06_master.h5 ##################################################### # 'cry-01-??_master.h5' are for 'full rotation helical' datasets ##################################################### # 60.0 deg. / datasets # 0.1 deg. / frame # 600 frames / datasets # 100 frames in each data file # ** The program could collect 'full rotation' helical dataset # but 60 deg. was set for this demonstration. ##################################################### hito-CPS1991-05//data/cry-01-00_data_000001.h5 hito-CPS1991-05//data/cry-01-00_data_000002.h5 hito-CPS1991-05//data/cry-01-00_data_000003.h5 hito-CPS1991-05//data/cry-01-00_data_000004.h5 hito-CPS1991-05//data/cry-01-00_data_000005.h5 hito-CPS1991-05//data/cry-01-00_data_000006.h5 hito-CPS1991-05//data/cry-01-00_master.h5溴化浸泡的溶菌酶晶体被用作标准晶体，用于评估由SPring-8 BL32XU开发的自动化数据收集系统ZOO。 尺寸介于10~100 µm的晶体被置于同一低温环（cryoloop）中一同进行低温冷冻，并采用ZOO系统中内置的「混合模式」完成数据处理。 光束尺寸：10（水平）×15（垂直） µm² 对该低温环完成二维光栅扫描后，HITO可分别针对完整旋转螺旋数据收集模式检测到1个晶体、针对部分螺旋模式检测到7个晶体，以及针对多小楔角模式检测到62个晶体。完整旋转螺旋、部分螺旋与小楔角数据集的总振荡角度分别为60°、40°与5°。 该晶体所属空间群为P4₃2₁2，平均晶胞参数为a=b=79.1，c=37.5。 在已发表的研究成果（Hirata等，待发表）中，使用KAMO软件将数据处理至1.50 Å分辨率。 <em><strong>【可用数据集】</strong></em> # 光束线： SPring-8 BL32XU # 波长： 0.9000 Å # 探测器： EIGER X 9M # ZOO模式: 混合模式（HITO） ##################################################### # 多小楔角数据集 # 'CPS1991-05-multi_master.h5' 管理62个数据集 ##################################################### # 5.0 deg. / 数据集 # 0.1 deg. / 帧 # 50 帧 / 数据集 # 100 帧 / 每个数据文件 ##################################################### hito-CPS1991-05//data/CPS1991-05-multi_data_000001.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000002.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000003.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000004.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000005.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000006.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000007.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000008.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000009.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000010.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000011.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000012.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000013.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000014.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000015.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000016.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000017.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000018.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000019.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000020.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000021.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000022.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000023.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000024.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000025.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000026.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000027.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000028.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000029.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000030.h5 hito-CPS1991-05//data/CPS1991-05-multi_data_000031.h5 hito-CPS1991-05//data/CPS1991-05-multi_master.h5 ##################################################### # 'cry-00-??_master.h5' 对应「部分螺旋」数据集 ##################################################### # 40.0 deg. / 数据集 # 0.1 deg. / 帧 # 400 帧 / 数据集 # 100 帧 / 每个数据文件 ##################################################### hito-CPS1991-05//data/cry-00-00_data_000001.h5 hito-CPS1991-05//data/cry-00-00_data_000002.h5 hito-CPS1991-05//data/cry-00-00_data_000003.h5 hito-CPS1991-05//data/cry-00-00_data_000004.h5 hito-CPS1991-05//data/cry-00-00_master.h5 hito-CPS1991-05//data/cry-00-01_data_000001.h5 hito-CPS1991-05//data/cry-00-01_data_000002.h5 hito-CPS1991-05//data/cry-00-01_data_000003.h5 hito-CPS1991-05//data/cry-00-01_data_000004.h5 hito-CPS1991-05//data/cry-00-01_master.h5 hito-CPS1991-05//data/cry-00-02_data_000001.h5 hito-CPS1991-05//data/cry-00-02_data_000002.h5 hito-CPS1991-05//data/cry-00-02_data_000003.h5 hito-CPS1991-05//data/cry-00-02_data_000004.h5 hito-CPS1991-05//data/cry-00-02_master.h5 hito-CPS1991-05//data/cry-00-03_data_000001.h5 hito-CPS1991-05//data/cry-00-03_data_000002.h5 hito-CPS1991-05//data/cry-00-03_data_000003.h5 hito-CPS1991-05//data/cry-00-03_data_000004.h5 hito-CPS1991-05//data/cry-00-03_master.h5 hito-CPS1991-05//data/cry-00-04_data_000001.h5 hito-CPS1991-05//data/cry-00-04_data_000002.h5 hito-CPS1991-05//data/cry-00-04_data_000003.h5 hito-CPS1991-05//data/cry-00-04_data_000004.h5 hito-CPS1991-05//data/cry-00-04_master.h5 hito-CPS1991-05//data/cry-00-05_data_000001.h5 hito-CPS1991-05//data/cry-00-05_data_000002.h5 hito-CPS1991-05//data/cry-00-05_data_000003.h5 hito-CPS1991-05//data/cry-00-05_data_000004.h5 hito-CPS1991-05//data/cry-00-05_master.h5 hito-CPS1991-05//data/cry-00-06_data_000001.h5 hito-CPS1991-05//data/cry-00-06_data_000002.h5 hito-CPS1991-05//data/cry-00-06_data_000003.h5 hito-CPS1991-05//data/cry-00-06_data_000004.h5 hito-CPS1991-05//data/cry-00-06_master.h5 ##################################################### # 'cry-01-??_master.h5' 对应「完整旋转螺旋」数据集 ##################################################### # 60.0 deg. / 数据集 # 0.1 deg. / 帧 # 600 帧 / 数据集 # 100 帧 / 每个数据文件 # ** 本程序可采集「完整旋转」螺旋数据集，但本次演示中设置为60°总振荡角度。 ##################################################### hito-CPS1991-05//data/cry-01-00_data_000001.h5 hito-CPS1991-05//data/cry-01-00_data_000002.h5 hito-CPS1991-05//data/cry-01-00_data_000003.h5 hito-CPS1991-05//data/cry-01-00_data_000004.h5 hito-CPS1991-05//data/cry-01-00_data_000005.h5 hito-CPS1991-05//data/cry-01-00_data_000006.h5 hito-CPS1991-05//data/cry-01-00_master.h5