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Reconstructing Draft Genomes Using Genome Resolved Meta genomics Reveal Arsenic Metabolizing Genes and Secondary Metabolites in Fresh Water Lake in Eastern India - Main Data

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Figshare2019-04-30 更新2026-04-28 收录
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We have taken water sample during winter and rainy season from Rabindra sarovar lake located at south kolkata and did metagenomic analysis. For metagenome analysis we followed genome resolved metagenomics approach. In this method samples are assembled after sequencing, then assembled samples are binned using binning tool to recover genome bins(n=27). Here in this repository we have uploaded recovered genome bins (.fna) and their respective 16S (.fa) as well as protein sequences (.faa) with annotation. A newick tree (.nwk) which infer phylogenetic position of 27 bins is addded here. This tree can edit anytime using ITOLv.4.In-addition we have also uploaded assembled data of both season(.fasta).

本研究于冬季与雨季期间,从位于加尔各答南部的拉宾德拉萨罗瓦尔湖(Rabindra sarovar lake)采集水样,并开展宏基因组学分析。宏基因组分析采用基因组解析宏基因组学(genome resolved metagenomics)策略,具体流程为:先对测序获得的样本进行序列组装,随后利用分箱工具对组装序列进行分箱以回收基因组分箱,最终共得到27个基因组分箱(n=27)。本仓库已上传回收得到的基因组分箱文件(格式为.fna)、对应的16S rRNA序列文件(格式为.fa)以及带注释信息的蛋白质序列文件(格式为.faa);同时上传了用于推断27个基因组分箱系统发育位置的Newick格式系统发育树文件(格式为.nwk),该系统发育树可通过ITOLv.4随时进行编辑。此外,本仓库还上传了两个季节的样本组装序列数据(格式为.fasta)。

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2019-04-30
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