What miRNAs are expressed in the E14.5 mouse midfacial tissue?

Researching what miRNAs are expressed in the E14.5 mouse midfacial tissue. Methods: * 1 dissected maxillas (4 mg w/w per maxilla; 4.0 ug of purified miRNA) * 6 dissected palatal shelves (1.5-2 mg w/w per palatal shelves; 3.5 ug of purified miRNA) * miRNA extracted using the Norgen miRNA Purification Kit, miRNA libraries prepared by ligation of barcoded adapters on 5' ends of small-RNA sequences, adapter was ligated to the 3' ends * miRNA-seq Illumina HiSeq

本研究旨在探究E14.5小鼠面中部组织中表达的微小RNA（miRNAs）。方法：
* 1枚解剖分离的上颌骨样本（每枚样本重量为4 mg，纯化得到微小RNA 4.0 μg）
* 6块解剖分离的腭突样本（每块样本重量1.5~2 mg，纯化得到微小RNA 3.5 μg）
采用Norgen微小RNA纯化试剂盒（Norgen miRNA Purification Kit）提取微小RNA，通过在小RNA序列的5'端连接带条形码的接头，并在3'端连接接头的方式制备miRNA测序文库，随后使用Illumina HiSeq平台开展miRNA测序（miRNA-seq）。