Effect of oncogenic transformation on metabolism 04

The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 largeT and smallT antigens (SV40 ER). TIG3 TSM and TSR cells were transformed lines by cMyc or H-RAS(G12V) in TERT/SV40 expressing TIG-3, respectively.

本研究采用添加内标重组蛋白的多反应监测（Multiple Reaction Monitoring，MRM）分析，对代谢酶的表达水平进行定量检测。本次分析的样本包括人二倍体成纤维细胞、TIG-3细胞及其衍生细胞系（TS、TSM及TSR）。其中TIG-3 TS为通过表达hTERT（human telomerase reverse transcriptase，人端粒酶逆转录酶）以及SV40大T抗原、小T抗原（SV40 ER）构建的永生化细胞系；TIG-3 TSM与TSR细胞则分别为在表达TERT与SV40抗原的TIG-3细胞中，通过转入c-Myc或H-RAS(G12V)获得的转化细胞系。