Diverse effect of WWOX overexpression in SW480 colon cancer cell line.

The WWOX gene is a tumor suppressor probably involved in regulation of cell cycle and apoptosis and downregulated in variety of cancer types.However, its role in colon cancerogenesis is unknown. The aim of this study was to characterize how WWOX may be involved in colon cancerogenesis or cancer progression, how it influences the basic cancer cell features and modifies cell expression profile.Our observations suggest that in HT29 colon cancer cell line increased expression of WWOX may result in transition of cancer cells into more normal- like colon epithelium phenotype, on the other hand in SW480 WWOX revealed the well-known tumour suppressor properties. However, as the colon cancer is very heterogeneous disease, obtained discrepancies may reflect the known differences between cell lines and cancerogenesis pathway, which they undergone. SW480 colon cancer cells were stably transfected with WWOX cDNA. SW480 cells transfected with an empty vector served as a control. Total mRNA was isolated to look for gene-expression differences induced by the WWOX overexpression.

WWOX基因是一种肿瘤抑制基因，可能参与细胞周期与细胞凋亡的调控，且在多种癌症类型中表达下调。然而，其在结肠癌发生过程中的作用仍不明确。本研究旨在阐明WWOX可能通过何种方式参与结肠癌发生或癌症进展、影响癌细胞的基本特征，并改变细胞的基因表达谱。我们的观察结果显示，在HT29结肠癌细胞系中，WWOX表达上调可促使癌细胞向更接近正常结肠上皮的表型转化；而在SW480细胞中，WWOX则表现出经典的肿瘤抑制功能。鉴于结肠癌是一种高度异质性的疾病，本研究中观察到的差异或可反映不同细胞系及其所经历的癌变通路之间的固有差异。本研究将SW480结肠癌细胞稳定转染WWOX cDNA，以空载体转染的SW480细胞作为对照，提取总mRNA以探究WWOX过表达诱导的基因表达差异。