Metabarcoding data analysis of foliar fungal communities associated with 84 woody species.

These metabarcoding data describe foliar fungal communities collected on 84 woody species planted at the Arboretum des Barres (Nogent-sur-Vernisson, France, 47.83819 N , 2.75792 E). Following fungal ITS1 amplification with ITS1F-ITS2 primers, two runs of single-end 454 sequencing were performed (Roche GS Junior). In total, 90 samples were sequenced : 88 environmental samples (i.e. leaf samples), one technical replicate corresponding to the same leaf sample (collected on Fagus sylvatica) sequenced twice, and one negative control for DNA extraction. The 88 environmental samples correspond to 84 samples collected on trees in the Arboretum des Barres and 4 samples simultaneously collected on trees in the Pierroton Iresearch station (INRAe, Cestas, France, 44.73734 N ; 0.77492 W). Bioinformatics scripts as well as raw and filtered ASV Tables are provided. Protocols and preliminary analyses are described in Quention Baillavoine's Master thesis (https://corinnevacher.files.wordpress.com/2014/11/baillavoine-quentin-rapport-de-stage-m2-fet.pdf).

本元条形码（metabarcoding）数据集描述了采自法国巴尔植物园（Nogent-sur-Vernisson，法国，北纬47.83819°，东经2.75792°）种植的84种木本植物的叶际真菌群落。研究采用ITS1F-ITS2引物对真菌的内转录间隔区1（ITS1）进行扩增，随后依托罗氏GS Junior测序平台完成两轮单端454测序。本次测序共涵盖90份样本：88份环境样本（即叶片样本）、1份对应欧洲山毛榉（Fagus sylvatica）同一份叶片样本的技术重复样本（经两次测序），以及1份DNA提取阴性对照。88份环境样本中，84份采自巴尔植物园的树木，剩余4份同期采集自法国皮耶尔庞研究站（INRAe，塞斯塔斯，法国，北纬44.73734°，西经0.77492°）的树木。本数据集附带生物信息学脚本、原始及过滤后的扩增子序列变异（ASV，Amplicon Sequence Variant）表。相关实验方案与初步分析结果已在昆坦·巴亚沃万（Quention Baillavoine）的硕士学位论文中详细阐述（https://corinnevacher.files.wordpress.com/2014/11/baillavoine-quentin-rapport-de-stage-m2-fet.pdf）。