Global timeospatial map of RNA polymerase in Escherichia coli. Global timeospatial map of RNA polymerase in Escherichia coli

To investigate the relationship between RNA polymerase binding and transcription ChIP-seq on the common house-keeping SigmaD and RNA polymerase beta subunit were coupled with RNA-seq at various growth phases of Escherichia coli in rich media. Overall design: ChIP-seq combined with RNA-seq to map RNA polymerase binding and transcription

为探究RNA聚合酶（RNA polymerase）结合与转录过程的关联，本研究以富营养培养基中培养的大肠杆菌（Escherichia coli）为研究对象，在其不同生长阶段下，针对持家型SigmaD因子与RNA聚合酶β亚基开展染色质免疫共沉淀测序（ChIP-seq），并将测序结果与RNA测序（RNA-seq）数据进行整合分析。实验设计概述：通过染色质免疫共沉淀测序联合RNA测序，绘制RNA聚合酶结合位点与转录的相关图谱。