Bulk RNA-seq of Mouse Colonic Organoids: SATB2 and FOXD2KO CRISPR Knockout

CRISPR knock out the Satb2 or Foxd2 gene from mouse colonic organoids to validate core human and mouse large intestine stem cell enrich TFs. Overall design: Three genomic modified mouse colonic organoids (Ctrl, Foxd2 and Satb2 sgRNAs) were differentiated and collected for Bulk RNA-Seq Please note that the additional raw counts data (prior to normalization), which was generated from the process of generation of counts relative to genes using featureCounts with default parameters (unstranded mode), was included in the records on Nov 5, 2021.

本研究通过CRISPR技术对小鼠结肠类器官进行Satb2或Foxd2基因敲除，以验证人类与小鼠大肠干细胞中富集的核心转录因子（TFs）。
实验设计概述：共设置3组经基因组编辑的小鼠结肠类器官（对照组、Foxd2 sgRNA组与Satb2 sgRNA组），对各组进行分化培养后收集样本，开展批量RNA测序（Bulk RNA-Seq）。
请注意，本数据集于2021年11月5日补充上传了未经标准化处理的原始计数数据，该数据系通过使用featureCounts软件并采用默认参数（非链特异性模式）针对基因生成计数所得。