Bulk RNA-Seq of human neutrophils and monocytes isolated from healthy controls, G-CSF-treated donors, HSCT, PDAC and IPMN patients

We aimed to characterize transcriptome plasticity of human myeloid cells in response to stress induced myelopoiesis. We performed bulk RNA sequencing (RNA-Seq) analyses of normal density neutrophils (NDNs), low density neutrophils (LDNs), and monocytes isolated from the peripheral blood (PB) of healthy controls (n=19), of G-CSF-treated donors (n=17) as well as of patients receiving hematopoietic stem cell transplantation (HSC-T; n=8), patients with locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC; n=15) or intraductal papillary mucinous neoplasms (IPMN; n=14). We also analyzed neutrophil differentiation intermediates from bone marrow samples of controls (n=3) or HSC-T patients (n=7). We generated a total of 210 RNA-Seq samples from 73 individuals.

本研究旨在解析人髓系细胞对应激诱导髓系生成的转录组可塑性。本研究对多类样本开展批量RNA测序（bulk RNA sequencing，RNA-Seq）分析：样本涵盖从19名健康对照、17名经粒细胞集落刺激因子（G-CSF）处理的供者、8名接受造血干细胞移植（hematopoietic stem cell transplantation，HSC-T）的患者、15名局部进展或转移性胰腺导管腺癌（pancreatic ductal adenocarcinoma，PDAC）患者及14名导管内乳头状黏液性肿瘤（intraductal papillary mucinous neoplasms，IPMN）患者的外周血（peripheral blood，PB）中分离的正常密度中性粒细胞（normal density neutrophils，NDNs）、低密度中性粒细胞（low density neutrophils，LDNs）与单核细胞。此外，本研究还对3名健康对照及7名HSC-T患者的骨髓样本中的中性粒细胞分化中间态细胞进行了分析。本研究共计从73名受试者中获取了210份RNA-Seq测序样本。