Table_1_Analysis of PANoptosis-Related LncRNA-miRNA-mRNA Network Reveals LncRNA SNHG7 Involved in Chemo-Resistance in Colon Adenocarcinoma.xlsx

Colon adenocarcinoma (COAD) is one of the most common malignancies, and its metastatic lesions are the leading cause of death in COAD patients. PANoptosis is a recently identified pathway for programmed cell death implicated in developing COAD. Long non-coding RNAs (lncRNAs) are key regulators of cancer occurrence and progress. Although their function has captured much attention in COAD, the relationship between COAD metastasis-associated lncRNA expression and PANoptosis remains elusive. Therefore, this study aimed to explore the potential regulatory roles of metastasis- and PANoptosis-associated lncRNAs in COAD. Nine lncRNAs associated with metastasis and PANoptosis in COAD were identified from The Cancer Genome Atlas (TCGA) and GEO databases. Their functions were analyzed by multiple bioinformatics methods, and the lncRNA-miRNA-mRNA network was constructed. Multivariate Cox analysis identified one lncRNA (SNHG7) significantly related to COAD prognosis. Subsequent analyses showed its expression correlated with tumor stage and lymph node metastasis. Moreover, drug sensitivity analysis and in vitro experiments suggest that lncRNA SNHG7 contributes to drug resistance in COAD. In summary, lncRNA SNHG7 is a potential target for diagnosing and treating COAD and plays a crucial role in regulating apoptosis, metastasis, and drug resistance in COAD.

结肠腺癌（Colon adenocarcinoma, COAD）是最常见的恶性肿瘤之一，其转移灶是COAD患者死亡的首要原因。泛凋亡（PANoptosis）是近年发现的一种参与COAD发生发展的程序性细胞死亡通路。长链非编码RNA（Long non-coding RNAs, lncRNAs）是肿瘤发生与进展的关键调控因子。尽管lncRNAs在COAD中的功能已受到广泛关注，但COAD转移相关lncRNA的表达与泛凋亡之间的关联仍不明晰。因此，本研究旨在探讨转移与泛凋亡相关lncRNAs在COAD中的潜在调控作用。研究人员从癌症基因组图谱（The Cancer Genome Atlas, TCGA）及基因表达综合数据库（Gene Expression Omnibus, GEO）中筛选得到9个与COAD转移及泛凋亡相关的lncRNAs。通过多种生物信息学方法分析这些lncRNAs的功能，并构建了lncRNA-miRNA-mRNA调控网络。多变量Cox回归分析鉴定出一个与COAD预后显著相关的lncRNA（SNHG7）。后续分析表明，该lncRNA的表达水平与肿瘤分期及淋巴结转移密切相关。此外，药物敏感性分析及体外实验（in vitro experiments）结果显示，lncRNA SNHG7可促进COAD细胞产生耐药性。综上，lncRNA SNHG7是COAD诊断与治疗的潜在靶点，其在调控COAD的细胞凋亡、转移及耐药过程中发挥关键作用。