Effect of tRF-1-Ser on the gene expression of breast cancer cell MDA-MB-453

tRNA-derived small RNAs (tsRNA) are a new type of noncoding RNAs that can be mainly classified into two groups: tRFs (tRNA-derived fragments) and tiRNAs (tRNA halves). The abnormal expression of tsRNAs is known to play an important role in the biological progression of breast cancer. However, it's still unclear about the mechanism of tsRNAs in cancer. tRF-1-Ser is a tRF that is high expression in breast cancer and negatively regulated by 25(OH)D. Our study aims to find out the effect of tRF-1-Ser on breast cancer and explore the change of RNA in tRF-1-Ser knock-down breast cancer cells. Overall design: To explore the influence of tRF-1-Ser on breast cancer, we transfected MDA-MB-453 with shtRF-1-Ser by lentivirus and verified the efficiency of knock-down by qPCR. The groups that MDA-MB-453 transfected with shtRF-1-Ser or shNC are named lv_shtRF or lv_con. Each group was duplicated three times.

转运RNA衍生小RNA（tRNA-derived small RNAs, tsRNA）是一类新型非编码RNA，主要可分为tRNA衍生片段（tRNA-derived fragments, tRFs）与tRNA半分子（tRNA halves, tiRNAs）两大类。已有研究证实，tsRNA的异常表达在乳腺癌的生物学进程中发挥重要作用，但其在肿瘤中的具体作用机制仍不明确。tRF-1-Ser是一类在乳腺癌中呈高表达、且受25(OH)D负调控的tRF。本研究旨在阐明tRF-1-Ser对乳腺癌的影响，并探究tRF-1-Ser敲低的乳腺癌细胞内RNA的表达变化。
实验设计概述：为探究tRF-1-Ser对乳腺癌的调控作用，本研究通过慢病毒将shtRF-1-Ser转染至MDA-MB-453细胞，并采用qPCR验证敲低效率。将转染shtRF-1-Ser与阴性对照shNC的MDA-MB-453细胞组分别命名为lv_shtRF与lv_con，每组设置3次重复。