Environmental DNA data from the RV Investigator South-East Australian Marine Ecosystem Survey (SEA-MES) voyages (IN2023_V05 and IN2024_V03)

This data is from a CSIRO project which was funded through the National Environmental Science Program (NESP) Marine and Coastal Hub - Project 4.23: “Environmental DNA for measuring offshore marine biodiversity: what can DNA in water collected from the RV Investigator tell us?”

The project generated a set of high-quality eDNA-based biodiversity datasets from samples collected at broadly distributed offshore sites across Southeast Australia. The data deposited here is processed data from eDNA metabarcoding assays (i.e. tables with sampling information, sequence read counts and taxonomy derived from the sequences), the raw sequence data (over 180 million sequences) is available on NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA1263580: Environmental DNA measuring offshore marine biodiversity in southeast Australia (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1263580/).

The eDNA biodiversity information focussed on fish but includes sequence data from the full tree of life (from bacteria to vertebrates). Part of the purpose of data collection was to assess key technical aspects of eDNA data collection to refine sampling methods and provide guidance for best practices in future offshore eDNA research

Details of the project can be accessed here:
https://www.nespmarinecoastal.edu.au/project/4-23/

This final report is available on the NESP Marine and Coastal Hub website:
https://www.nespmarinecoastal.edu.au/publication/environmental-dna-for-measuring-offshore-marine-biodiversity-what-can-dna-in-water-collected-from-the-rv-investigator-tell-us/

本数据集源自澳大利亚联邦科学与工业研究组织（Commonwealth Scientific and Industrial Research Organisation, CSIRO）的一项科研项目，该项目由国家环境科学计划（National Environmental Science Program, NESP）海洋与海岸中心资助，项目编号为4.23，主题为"环境DNA用于近海海洋生物多样性监测：“调查者号”科考船（RV Investigator）采集的水样中的DNA能揭示哪些信息？"

该项目从澳大利亚东南部广泛分布的近海采样点位采集样本，生成了一系列基于环境DNA（eDNA）的高质量生物多样性数据集。

本次上传的数据为环境DNA宏条形码（metabarcoding）分析的处理后数据，即包含采样信息、序列读段计数以及由序列推导得到的分类学信息的表格；原始序列数据（总量超1.8亿条序列）已上传至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的BioProject PRJNA1263580条目下，相关主题为"澳大利亚东南部近海海洋生物多样性的环境DNA监测"，链接：https://www.ncbi.nlm.nih.gov/

本次eDNA生物多样性数据以鱼类为核心监测对象，但同时涵盖了从细菌到脊椎动物的全生命之树各分支的序列数据。

本次数据采集的核心目标之一为评估eDNA数据采集流程中的关键技术环节，以优化采样方法，并为未来近海eDNA研究的最佳实践提供指导性建议。

该项目的详细信息可通过以下链接查阅：https://www.nespmarinecoastal.edu.au/project/4-23/

本项目总结报告将发布于NESP海洋与海岸中心官方网站：www.nespmarinecoastal.edu.au