Continuous in vitro culture of Cryptosporidium parvum

Cryptosporidium parvum (isolate BGF-T0) was cultured in a hollow fiber bioreactor as described in Morada et al., 2016, Int J Parasitol 46:21-29. At 20 months 108 oocysts were collected and assigned isolate BGF-T20HF. Genome sequences were generated at the Beijing Genomics Institute using 1 ug of purified DNA and compared with the parent BGF-T0.

微小隐孢子虫（Cryptosporidium parvum）分离株BGF-T0按照Morada等人2016年发表于《国际寄生虫学期刊（Int J Parasitol）》第46卷第21-29页的方法，在中空纤维生物反应器中培养。培养20个月后，研究人员收集到108个卵囊，并将其命名为分离株BGF-T20HF。北京基因组研究所（Beijing Genomics Institute）使用1微克纯化的DNA完成了该分离株的基因组测序，并将测序结果与亲本分离株BGF-T0进行了比对。