Additional file 1: Figure S1. of CD80 down-regulation is associated to aberrant DNA methylation in non-inflammatory colon carcinogenesis
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https://figshare.com/articles/dataset/Additional_file_1_Figure_S1_of_CD80_down-regulation_is_associated_to_aberrant_DNA_methylation_in_non-inflammatory_colon_carcinogenesis/4472960
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MSP analysis. Representative results of methylation-specific PCR of APC, CDH13, MGMT, MLH1 and RUNX3 in colorectal mucosa specimen. The presence of a visible PCR product indicates the presence of unmethylated and methylated genes as shown; the EpiTect PCR Control DNA Set (Qiagen) was used as the positive control (+) for the methylated and unmethylated genes. (ZIP 1770Â kb)
甲基化特异性PCR(Methylation-Specific PCR,MSP)分析:针对结直肠黏膜标本中APC、CDH13、MGMT、MLH1及RUNX3基因的甲基化特异性PCR代表性检测结果。如所示,出现可见PCR产物即代表存在未甲基化与甲基化的对应基因;本实验采用EpiTect PCR对照DNA试剂盒(Qiagen)作为甲基化及未甲基化基因的阳性对照(+)。(ZIP 1770 KB)
创建时间:
2016-12-16



