Establishment and characterization of patient-derived xenograft from leptomeningeal spread of a rare pediatric anaplastic pleomorphic xanthoastrocytoma (PXA) bearing a CDC42SE2-BRAF fusion.

To establish and to characterize patient derived preclinical model of rare pediatric glioma, anaplastic pleomorphic xanthoastrocytoma. The model was derived from tumor found at leptomeningeal spread site. we perform multi omics analysis including in this file is RNA seq to asses molecular fidelity of the patient derived model ( xenograft and xenoline) compared to original tumor from patient. Overall design: Comparative gene expression profiling analysis of RNA-seq data for xenograft passage 1 (PDX1281) and 2 (PDX1282), xenoline (X128), normal human astrocyte and patient tumor (Pttumor)

本研究旨在建立并表征源自罕见儿童胶质瘤——间变性多形性黄色星形细胞瘤（anaplastic pleomorphic xanthoastrocytoma）的患者来源临床前模型。该模型取自软脑膜播散部位的肿瘤组织。本研究开展多组学分析，其中包含RNA测序（RNA-seq），以评估患者来源模型（异种移植瘤模型与异种细胞系模型）相较于患者原发肿瘤的分子保真度，相关分析数据收录于本文件中。整体实验设计：针对第1代异种移植瘤（PDX1281）、第2代异种移植瘤（PDX1282）、异种细胞系（X128）、正常人类星形胶质细胞以及患者原发肿瘤（Pttumor）的RNA-seq数据进行比较基因表达谱分析。