Wild-type C1845-induced gene expression

Cells were infected for four hours with Afa/Dr DAEC wild-type strain C1845 and total RNA extracted. Selected gene expression was assayed by q-PCR. The oligonucleotides used in this study were designed by Applied Biosystems, the manufacturer's references are given in brackets. Abbreviations are as follows: VEGF: Vascular Endothelial Growth Factor, IL8: interleukin 8, PlGF: Placental growth factor, TGFβ R2: Transforming growth factor Receptor 2, EGF R1: Epithelial growth factor Receptor 1. Results (means±SD) are representative of independent experiments (where n represents the number of experiments). *, p将细胞与Afa/Dr DAEC野生型菌株C1845共感染4小时，随后提取总RNA。采用q-PCR对选定基因的表达水平进行检测。本研究使用的寡核苷酸由应用生物系统公司（Applied Biosystems）设计，其厂商参考编号标注于括号内。缩写定义如下：血管内皮生长因子（Vascular Endothelial Growth Factor, VEGF）、白细胞介素8（interleukin 8, IL8）、胎盘生长因子（Placental growth factor, PlGF）、转化生长因子β受体2（Transforming growth factor Receptor 2, TGFβ R2）以及表皮生长因子受体1（Epithelial growth factor Receptor 1, EGF R1）。实验结果以均值±标准差（mean±SD）呈现，代表独立重复实验的结果（其中n代表实验重复次数）。*，p