Molecular interactions at the bovine embryo–endometrial epithelium interface

We aimed to interrogate the embryo–maternal interface for embryo-induced changes in the global transcriptome of bovine endometrial epithelial cells (bEECs). In vitro-produced day-5.5 morulae were cultured in juxtacrine apposition (Contact+) with bEECs, or in transwells (Contact?), for 48 h. A group of bEECs cultured in the absence of embryos (Control bEEC) was also included. After co-culture, bEECs were recovered for transcriptome analysis. Overall design: Bovine endometrial epithelial cells (bEECs) were co-cultured in juxtacrine (Contact+) or non-juxtacrine (Contact-) apposition with in vitro-derived morulae for 48 h. bEECs transcriptomes were sequenced (NGS) on the BGISEQ-500 platform.

本研究旨在解析胚胎-母体界面，探究胚胎诱导的牛子宫内膜上皮细胞（bovine endometrial epithelial cells，bEECs）全局转录组变化。将体外制备的第5.5天桑椹胚，分别与bEECs以邻位并置方式（Contact+组）共培养，或置于Transwell小室中（Contact−组）共培养48小时；同时设置未添加胚胎的bEECs培养组作为对照（Control bEEC组）。共培养结束后，回收bEECs用于转录组分析。整体实验设计如下：将牛子宫内膜上皮细胞（bEECs）与体外来源的桑椹胚，分别以邻位接触（Contact+）或非邻位接触（Contact−）的方式共培养48小时，随后采用BGISEQ-500平台对bEECs转录组进行下一代测序（NGS）。