Gene expression analysis of EpH4 cell monolayers following TGF-β3 treatment. Mus musculus

EpH4 cell monolayers were treated with 1 ng/ml TGF-β3 or vehicle for 24 hours. The experiment was performed three independent times, with 3 wells per condition in each experiment. Keywords: Expression profiling by array Overall design: Each sample represents pooled RNA from three wells of a single experiments. N=3 experiments. Gene expression was analyzed using Affymetrix Mouse Gene 1.1 ST Array Strips and the GeneAtlas System.

将EpH4细胞单层用1 ng/ml的转化生长因子-β3（TGF-β3）或溶剂对照处理24小时。该实验独立重复三次，每次实验中每个处理组设置3个复孔。关键词：基于芯片的表达谱分析。实验设计概述：每份样本均取自单次实验中3个复孔的混合RNA，共开展3次独立实验（N=3）。基因表达分析采用Affymetrix小鼠基因1.1 ST 芯片条带及GeneAtlas系统完成。