ING3 and ING4 immunoexpression and their relation to the development of benign odontogenic lesions

Abstract The Inhibitor of Growth (ING) gene family is a group of tumor suppressor genes that play important roles in cell cycle control, senescence, DNA repair, cell proliferation, and apoptosis. However, inactivation and downregulation of these proteins have been related in some neoplasms. The present study aimed to evaluate the immunohistochemical profiles of ING3 and ING4 proteins in a series of benign epithelial odontogenic lesions. Methods: The sample comprised of 20 odontogenic keratocysts (OKC), 20 ameloblastomas (AM), and 15 adenomatoid odontogenic tumors (AOT) specimens. Nuclear and cytoplasmic immunolabeling of ING3 and ING4 were semi-quantitatively evaluated in epithelial cells of the odontogenic lesions, according to the percentage of immunolabelled cells in each case. Descriptive and statistics analysis were computed, and the p-value was set at 0.05. Results: No statistically significant differences were found in cytoplasmic and nuclear ING3 immunolabeling among the studied lesions. In contrast, AOTs presented higher cytoplasmic and nuclear ING4 labeling compared to AMs (cytoplasmic p-value = 0.01; nuclear p-value摘要 生长抑制因子（Inhibitor of Growth, ING）基因家族是一类在细胞周期调控、细胞衰老、DNA修复、细胞增殖及细胞凋亡过程中发挥重要作用的肿瘤抑制基因家族。然而，此类蛋白的失活与表达下调已在多种肿瘤性疾病中被发现与病变发生相关。本研究旨在评估ING3与ING4蛋白在一系列良性上皮性牙源性病变中的免疫组化表达谱。方法：本研究样本包含20例牙源性角化囊性瘤（odontogenic keratocysts, OKC）、20例成釉细胞瘤（ameloblastomas, AM）及15例腺瘤样牙源性肿瘤（adenomatoid odontogenic tumors, AOT）标本。根据每例标本中免疫标记阳性细胞的占比，对牙源性病变上皮细胞中ING3与ING4的细胞核及细胞质免疫标记进行半定量评估。本研究开展了描述性与统计学分析，设定P值阈值为0.05。结果：在所研究的三类病变中，ING3的细胞质与细胞核免疫标记水平均未发现统计学显著性差异。与之相反，腺瘤样牙源性肿瘤的细胞质与细胞核ING4标记水平均高于成釉细胞瘤（细胞质P值=0.01；细胞核P值