Knockout PTTG1 HCT116 cells

To identify potential PTTG1-targeted genes, total RNA from PTTG1+/+ and PTTG1-/- HCT 116 cells was isolated using the RNeasy Kit (Qiagen) and analyzed using Illumina microarrays (HumanHT-12_V4). Raw data were processed according to the manufacturer’s standard procedure and further analyzed using Partek 6.5. PTTG1-/- and parent HCT116 cells. Experiment is performed in triplicate

为鉴定潜在的垂体肿瘤转化基因1（PTTG1）靶向基因，研究人员从PTTG1纯合野生型（PTTG1+/+）与纯合敲除型（PTTG1-/-）HCT 116细胞中提取总RNA，采用RNeasy试剂盒（RNeasy Kit，Qiagen）完成提取后，使用Illumina基因芯片（Illumina microarrays，HumanHT-12_V4）进行表达分析。原始数据按照试剂盒厂商的标准流程进行预处理，并借助Partek 6.5软件开展进一步数据分析。本实验以PTTG1敲除型（PTTG1-/-）与亲本HCT 116细胞为实验材料，且设置三次生物学重复。