Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogramme the host immune response during persistent infection [RNA-seq]. Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogramme the host immune response during persistent infection [RNA-seq]

scRNA-seq of CD45+ leukocytes isolated from WT and triple mutant-infected mice identified several genes with concordant changes in H3Ac status and transcriptional expression. Overall design: Live, CD45+ leukocytes were isolated by FACs from implant-associated tissue at day 14-post infection for scRNA-seq. While these samples were prepared and sequenced for single-cell RNA-seq, they were analyzed as bulk RNA for this study. All raw data for a single-cell analysis have been included.

从野生型（Wild Type, WT）和三重突变体感染小鼠中分离的CD45+白细胞的单细胞RNA测序（scRNA-seq）分析，鉴定出多个组蛋白H3乙酰化（H3Ac）修饰状态与转录表达水平呈现一致性变化的基因。
整体实验设计：于感染后第14天，通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS，原文标注为FACs）从植入物相关组织中分离活的CD45+白细胞用于scRNA-seq。尽管本研究已按照单细胞RNA测序的流程完成这些样本的建库与测序，但本次分析将其作为批量RNA样本开展。所有用于单细胞分析的原始数据均已纳入本数据集。