Italian CryoEM Bag II (ICB-II)

This BAG application builds on the development of single particle cryoEM in the Italian Structural Biology community, as in Italy there is no top-end instrument isopen to rhe structural biology community.
The groups in this application have acquired know-how in cryoEM through international collaborations or by acquiring medium-level in-house equipment. Access tothe CM01 beamline at the ESRF since August 2020 has allowed the development of projects from groups that do not have in-house equipment that resulted inpaper in publication on Nucleic Acid Research NAR.
We now have six screening microscopes in Italy and two more are being funded, making the access to CM01 more demanded and relevant for high-resolution
data and, consequently high profile publications

本BAG申请依托意大利结构生物学界的单粒子冷冻电子显微镜（cryoEM）技术发展而提出，因意大利目前尚无面向结构生物学领域开放的高端冷冻电镜设备。
本申请涉及的各研究团队，通过国际合作或购置中型自主科研设备的路径，掌握了冷冻电镜相关技术诀窍。自2020年8月起，团队得以使用欧洲同步辐射光源（ESRF）的CM01光束线，这使得无自主设备的研究团队得以开展相关科研项目，并最终在《核酸研究》（Nucleic Acid Research, NAR）期刊发表多篇学术论文。
目前意大利境内已有6台筛选级冷冻电镜，另有两台设备的资助申请已获批，这使得CM01光束线的使用需求愈发迫切，其对于获取高分辨率实验数据、进而发表高影响力学术成果均具有关键支撑作用。