The exome capture samples are from hybrids between each accession from our panel and Morex. We have pre-existing exome capture and/or whole-genome shotgun data for all of the parents, but wanted DNA information from the hybrids in order to verify SNPs between parental DNA and RNA-seq samples. The sample names include a number and the accession name. All hybrids are between the named accession and Morex. DNA was harvested from 1-week old seedling tissue using the Qiagen DNeasy kit. DNA enriched using the barley exome capture platform was sequenced using 100 bp paired-end Illumina Hi-Seq sequencing. The Contribution of Cis- And Trans-Acting Variants to Gene Regulation in Wild and Domesticated Barley Under Cold Stress and Control Conditions

The exome capture samples are from hybrids between each accession from our panel and Morex. We have pre-existing exome capture and/or whole-genome shotgun data for all of the parents, but wanted DNA information from the hybrids in order to verify SNPs between parental DNA and RNA-seq samples. The sample names include a number and the accession name. All hybrids are between the named accession and Morex. DNA was harvested from 1-week old seedling tissue using the Qiagen DNeasy kit. DNA enriched using the barley exome capture platform was sequenced using 100 bp paired-end Illumina Hi-Seq sequencing

本研究的外显子组捕获样本，均来自本研究材料组中每份种质材料（accession）与大麦品种Morex的杂交后代。我们已获取所有亲本的外显子组捕获（exome capture）和/或全基因组鸟枪法测序（whole-genome shotgun）数据，但为验证亲本DNA与RNA测序（RNA-seq）样本间的单核苷酸多态性（Single Nucleotide Polymorphisms，简称SNPs），仍需获取杂交后代的DNA信息。样本名称由数字与对应种质材料名称组合而成，所有杂交后代均为该命名种质材料与Morex的杂交子代。本实验采用凯杰（Qiagen）DNeasy试剂盒，从1周龄幼苗组织中提取基因组DNA；利用大麦外显子组捕获平台对DNA进行富集后，采用100 bp双端测序策略，通过Illumina HiSeq测序平台完成测序。