Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells

NOD mice were treated with PBS or OX40L and Jagged-1 proteins once a week for three weeks. A week after final treatment CD4+CD25- Teff cells and CD4+CD25hi Treg cells were sorted by FACS. Genomic DNA isolated from sorted cells and differences in DNA methylation pattern in these cell types between PBS and OX40L-Jagged-1 treated mice was analyzed by whole genome bi-sulfite sequencing. Examination of methylation pattern in Teff vs Treg cells between PBS and OX40L-JAG1 mice.

将非肥胖糖尿病小鼠（NOD mice）分别以磷酸盐缓冲液（PBS）、OX40L蛋白与Jagged-1蛋白进行每周一次的处理，连续三周。末次处理一周后，通过荧光激活细胞分选术（FACS）分离得到CD4+CD25- 效应T细胞（Teff cells）与CD4+CD25hi 调节性T细胞（Treg cells）。从分选所得细胞中提取基因组DNA，采用全基因组亚硫酸氢盐测序，分析PBS处理组与OX40L-Jagged-1处理组小鼠中上述两类细胞的DNA甲基化模式差异。对PBS处理组与OX40L-JAG1处理组小鼠中效应T细胞与调节性T细胞的甲基化模式进行比较分析。