GI RNA-seq

Holstein cattle are one of the major ruminants that supply milk and dairy products to the human diet and agribusiness. To investigate the molecular basis of the adaption of dairy cattle for milk synthesis, we used RNA-seq to profile gene expression patterns in epithelial cells derived from three gastrointestinal tract tissues, including the rumen, duodenum, and colon at 8 time points (Days 3, 14, 28, 45, 120, 220, 305, and the dry period). Overall, this resource will provide comprehensive insights into cattle lactation and reveal the specific characteristics of gastrointestinal tract tissues to understand the complexity of transcriptomic activities during lactation.荷斯坦奶牛（Holstein cattle）是为人类膳食及农业综合企业供给牛乳与乳制品的核心反刍动物类群之一。为解析奶牛泌乳合成适应性的分子基础，本研究采用RNA测序（RNA-seq）技术，对瘤胃（rumen）、十二指肠（duodenum）、结肠（colon）3种胃肠道组织来源的上皮细胞，在8个时间节点（第3、14、28、45、120、220、305日及干奶期）的基因表达模式开展了转录组分析。总体而言，本数据集将为奶牛泌乳相关研究提供全面的认知视角，并揭示胃肠道组织的特异性特征，助力阐明泌乳过程中转录组活动的复杂调控机制。