Lung Natural Helper cells

Natural Helper cells constitute a unique lineage of Th2-cytokine producting innate lymphocytes, here we characterize the gene expression profile of non-stimulated or PMA/ionomycin-stimulated Natural Helper cells from naive C57Bl/6 mouse lungs. We used microarrays to detail the gene expression profile of stimulated and unstimulated lung Natural Helper cells in mice. Lineage(-)Sca-1(+)c-Kit(-/low)CD127(+)CD25(+) Lung Natural Helper cells were purified from naive 6-8 week old B6 mice by FACS. RNA was extracted immediately from un-stimulated Natural Helper cells after FACS. Stimulated Natural Helper cells were cultured with PMA/ionomycin for 3 days followed by RNA extraction.天然辅助细胞（Natural Helper cells）是一类独特的分泌Th2型细胞因子的固有淋巴细胞谱系。本研究对来自未致敏C57BL/6小鼠肺部的未刺激或经PMA/离子霉素刺激的天然辅助细胞的基因表达谱进行了表征。我们采用微阵列技术，详细解析了小鼠肺部刺激组与未刺激组天然辅助细胞的基因表达谱。通过荧光激活细胞分选（FACS），从6~8周龄未致敏的C57BL/6小鼠体内纯化得到表型为Lineage(-)Sca-1(+)c-Kit(-/low)CD127(+)CD25(+)的肺部天然辅助细胞。未刺激组的天然辅助细胞在分选完成后即刻提取RNA；刺激组的天然辅助细胞则经PMA/离子霉素培养3天后，再进行RNA提取。