RNAseq analysis of blood from respiratory viruses-infected patients and healthy controls

The objective of this study was to understand the shared and unique elements of the host transcriptional response to different viral pathogens. We identified 162 subjects in the US and Sri Lanka with infections due to influenza, enterovirus/rhinovirus, human metapneumovirus, dengue virus, cytomegalovirus, Epstein Barr Virus, or adenovirus. Our dataset allowed us to identify common pathways at the molecular level as well as virus-specific differences in the host immune response. Conserved elements of the host response to these viral infections high-lighted the importance of interferon pathway activation. However, the magnitude of the re-sponses varied between pathogens. We also identified virus-specific responses to influenza, enterovirus/rhinovirus, and dengue infections. Overall design: Peripheral blood was collected from patients, RNA was isolated and sequenced.

本研究旨在探究宿主对不同病毒病原体产生转录应答时的共有与独有特征。我们在美国与斯里兰卡共纳入162名感染患者，所涉病原体包括流感病毒、肠道病毒/鼻病毒、人偏肺病毒、登革病毒、巨细胞病毒、EB病毒（Epstein Barr Virus）以及腺病毒。本数据集可用于在分子层面鉴定宿主免疫应答的共通通路与病毒特异性差异。宿主对这类病毒感染的应答保守特征凸显了干扰素通路激活的重要性，但不同病原体引发的应答强度存在差异。我们还鉴定出针对流感、肠道病毒/鼻病毒以及登革病毒感染的病毒特异性应答。
实验整体设计如下：采集患者外周血，分离RNA并进行测序。