Table_2_The causal correlation between gut microbiota abundance and pathogenesis of cervical cancer: a bidirectional mendelian randomization study.docx

BackgroundObservational studies and animal experiments suggested potential relevance between gut microbiota (GM) and cervical cancer (CC), but the relevance of this association remains to be clarified.
MethodsWe performed a two-sample bidirectional Mendelian randomization (MR) analysis to explore whether there was a causal correlation between GM and CC, and the direction of causality.
ResultsIn primary outcomes, we found that a higher abundance of class Clostridia, family Family XI, genus Alloprevotella, genus Ruminiclostridium 9, and order Clostridiales predicted higher risk of CC, and a higher abundance of class Lentisphaeria, family Acidaminococcaceae, genus Christensenellaceae R7 group, genus Marvinbryantia, order Victivallales, phylum Actinobacteria, and phylum Lentisphaerae predicted lower risk of CC. During verifiable outcomes, we found that a higher abundance of class Methanobacteria, family Actinomycetaceae, family Methanobacteriaceae, genus Lachnospiraceae UCG 010, genus Methanobrevibacter, order Actinomycetales, and order Methanobacteriales predicted a higher risk of CC, and a higher abundance of family Streptococcaceae, genus Dialister, and phylum Bacteroidetes predicted a lower risk of CC, and vice versa.
ConclusionOur study implied a mutual causality between GM and CC, which provided a novel concept for the occurrence and development of CC, and might promote future functional or clinical analysis.

背景 观察性研究与动物实验均提示肠道菌群（gut microbiota, GM）与宫颈癌（cervical cancer, CC）之间存在潜在关联，但二者关联的具体相关性仍有待阐明。方法 本研究采用两样本双向孟德尔随机化（two-sample bidirectional Mendelian randomization, MR）分析，探讨肠道菌群与宫颈癌之间是否存在因果关联，以及因果关联的方向。结果 在主要结局分析中，本研究发现：梭菌纲（Clostridia）、Family XI科（Family XI）、异普雷沃菌属（Alloprevotella）、瘤胃梭菌属9（Ruminiclostridium 9）以及梭菌目（Clostridiales）的丰度升高，可预测宫颈癌风险升高；而透镜菌纲（Lentisphaeria）、氨基酸球菌科（Acidaminococcaceae）、克里斯滕森菌科R7群（Christensenellaceae R7 group）、马文布莱恩特菌属（Marvinbryantia）、胜利杆菌目（Victivallales）、放线菌门（Actinobacteria）以及透镜菌门（Lentisphaerae）的丰度升高，可预测宫颈癌风险降低。在验证性结局分析中，本研究发现：产甲烷菌纲（Methanobacteria）、放线菌科（Actinomycetaceae）、产甲烷杆菌科（Methanobacteriaceae）、毛螺菌科UCG 010属（Lachnospiraceae UCG 010）、甲烷短杆菌属（Methanobrevibacter）、放线菌目（Actinomycetales）以及产甲烷杆菌目（Methanobacteriales）的丰度升高，可预测宫颈癌风险升高；而链球菌科（Streptococcaceae）、嗜胆菌属（Dialister）以及拟杆菌门（Bacteroidetes）的丰度升高，可预测宫颈癌风险降低，反之亦然。结论 本研究提示肠道菌群与宫颈癌之间存在双向因果关联，为宫颈癌的发生与发展提供了全新的研究视角，或可推动后续功能学或临床相关研究的开展。