How Does Hydrophobicity Change of Nonionic Surfactant Influence the Co-adsorption of Monoclonal Antibody and Surfactant at the Amphiphilic Self-assembled Interface?

We have previously found that nonionic surfactants, such as Polysorbate 80 (commonly denoted as PS80 or Tween 80), could form a monolayer on amphiphilic UV-TMOS treated surface, greatly inhibiting mAb from further adsorption. We speculate that the hydrophilic heads of PS80, which are adsorbed on the outside of the UV-TMOS interface, prevent further adsorption of the mAbs. This aroused our interest to investigate whether the hydrophobicity of surfactants, especially headgroup size, would impact the co-adsorption of mAb and surfactant.

我们此前已发现，非离子表面活性剂（如聚山梨酯80（Polysorbate 80），通常简称为PS80或Tween 80）可在两亲性UV-TMOS处理表面形成单分子层，显著抑制单克隆抗体（mAb）的进一步吸附。我们推测，吸附于UV-TMOS界面外侧的PS80亲水头部基团，可阻碍单克隆抗体的后续吸附。这一发现引发了我们的研究兴趣，旨在探究表面活性剂的疏水性——尤其是其头部基团尺寸——是否会影响单克隆抗体与表面活性剂的共吸附行为。