MOESM1 of Combining transcriptomics and metabolomics to reveal the underlying molecular mechanism of ergosterol biosynthesis during the fruiting process of Flammulina velutipes

Additional file 1: Table S1. The statistics of RNA-Seq data at different developmental stages of F. velutipes. Figure S1. The DEG statistics at different stages of F. velutipes. Figure S2. GO functional annotation and classification of DEGs during T1 of F. velutipes. Figure S3. GO functional annotation and classification of DEGs during T2 of F. velutipes. Figure S4. Differential expression profiles of nine DEGs at different developmental stages of F. velutipes. GAPDH and β-actin were used as internal controls. There were three biological replicates and three technical replicates for each gene. Table S2. The results of differential ions identification at different developmental stages of F. velutipes. Table S3. Identified metabolites in the ergosterol biosynthesis during T1 of F. velutipes. Table S4. Identified metabolites in the ergosterol biosynthesis during T2 of F. velutipes. Figure S5. Verification results of the end product ergosterol in metabolomics. (A) LC-MS spectrum of the ergosterol standard. (B) LC-MS spectrum of ergosterol from F. velutipes. Their corresponding m/z and retention time (RT) are indicated in the figure. Excel S1. The profiles of metabolites and gene expression at three different developmental stages of F. velutipes using Pearson’s correlation coefficient. Excel S2. Network analysis results of metabolites and genome-wide genes at three different developmental stages of F. velutipes by Cytoscape. Table S5. Primer sequences of DEGs related to ergosterol biosynthesis in F. velutipes.附加文件1：表S1。金针菇（Flammulina velutipes，以下简称F. velutipes）不同发育阶段的RNA测序（RNA-Seq）数据统计信息。 图S1。F. velutipes不同发育阶段的差异表达基因（Differentially Expressed Genes，以下简称DEG）统计情况。 图S2。F. velutipes T1阶段DEG的基因本体（Gene Ontology，以下简称GO）功能注释及分类。 图S3。F. velutipes T2阶段DEG的GO功能注释及分类。 图S4。F. velutipes不同发育阶段9个DEG的差异表达谱。以甘油醛-3-磷酸脱氢酶（GAPDH）和β-肌动蛋白（β-actin）作为内参基因，每个基因设置3次生物学重复与3次技术重复。 表S2。F. velutipes不同发育阶段差异离子鉴定结果。 表S3。F. velutipes T1阶段麦角固醇生物合成途径中鉴定得到的代谢物。 表S4。F. velutipes T2阶段麦角固醇生物合成途径中鉴定得到的代谢物。 图S5。代谢组学中终产物麦角固醇的验证结果：(A) 麦角固醇标准品的液相色谱-质谱（Liquid Chromatography-Mass Spectrometry，以下简称LC-MS）谱图；(B) F. velutipes来源麦角固醇的LC-MS谱图，图中标注了二者对应的质荷比（m/z）与保留时间（RT）。 Excel文件S1。基于皮尔逊相关系数的F. velutipes 3个不同发育阶段代谢物与基因表达谱。 Excel文件S2。借助Cytoscape软件，对F. velutipes 3个不同发育阶段的代谢物与全基因组基因进行网络分析的结果。 表S5。F. velutipes麦角固醇生物合成相关DEG的引物序列。